{"id":973,"date":"2013-03-05T22:17:52","date_gmt":"2013-03-05T14:17:52","guid":{"rendered":"http:\/\/www.leadingtec.cn\/?p=973"},"modified":"2014-06-30T12:17:32","modified_gmt":"2014-06-30T04:17:32","slug":"bg11-n-n-medium","status":"publish","type":"post","link":"https:\/\/www.leadingtec.cn\/bg11-n-n-medium.html","title":{"rendered":"BG11\u57f9\u517b\u57fa\uff08+N\/-N\uff09"},"content":{"rendered":"

\u53c2\u8003\u6587\u732e\uff1aStanier,\u00a0R.Y.,\u00a0Kunisawa,\u00a0R.,\u00a0Mandel,\u00a0M.&\u00a0Cohen-Bazire,\u00a0G.1971.\u00a0Purification\u00a0and\u00a0properties\u00a0of\u00a0unicellular\u00a0blue-green\u00a0algae\u00a0(Order\u00a0Chroococcales).\u00a0<\/span>Bacteriol.\u00a0Rev.\u00a035:\u00a0171-205.<\/p>\n

\u4e0a\u6d77\u5149\u8bed\u751f\u7269\u79d1\u6280\u6709\u9650\u516c\u53f8\u63d0\u4f9b\u914d\u7f6e\u597d\u7684bg11\u57f9\u517b\u6db2<\/a>\uff0c\u5982\u9700\u8282\u7701\u65f6\u95f4\u8bf7\u8054\u7cfb\u6211\u4eec<\/a>\u3002<\/p>\n

\u51c6\u5907\u50a8\u5907\u6db2Stocks\u89c1\u4e0b\u8868\uff1a<\/span><\/strong><\/p>\n\n\n\n\n\n\n
Stock1<\/strong><\/td>\n\u8bd5\u5242<\/strong><\/td>\nper\u00a01000mL\u00a0<\/strong><\/td>\nper\u00a0500mL<\/strong><\/td>\n<\/tr>\n
<\/td>\nNaNO3(+N)\/NaCl(-N)<\/td>\n30.0g<\/td>\n15.0g<\/td>\n<\/tr>\n
1<\/td>\nK2HPO4\u00a0\/\u00a0K2HPO4\u00b73H2O<\/td>\n0.8g\u00a0\/\u00a01.05g<\/td>\n0.4g\u00a0\/\u00a00.53g<\/td>\n<\/tr>\n
2<\/td>\nMgSO4\u00b77H2O<\/td>\n1.5g<\/td>\n0.75g<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n
Stock2<\/strong><\/td>\n\u8bd5\u5242<\/strong><\/td>\nper\u00a0100mL\u00a0<\/strong><\/td>\nper\u00a0200mL<\/strong><\/td>\n<\/tr>\n
3<\/td>\n\u67e0\u6aac\u9178\/<\/span>\u4e00\u6c34\u5408\u67e0\u6aac\u9178<\/span>C6H8O7<\/td>\n0.30g\/0.33g<\/td>\n0.60\/0.66g<\/td>\n<\/tr>\n
3<\/td>\n\u67e0\u6aac\u9178\u94c1\u94f5\uff1aFeC6H5O7.NH4OH<\/td>\n0.30g<\/td>\n0.60g<\/td>\n<\/tr>\n
3<\/td>\nNa2EDTA\/Na2EDTA\u00b72H2O<\/td>\n0.05g\/0.055g<\/td>\n0.10g\/0.11g<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n
Stock3<\/strong><\/td>\n\u8bd5\u5242<\/strong><\/td>\nper\u00a0100mL\u00a0<\/strong><\/td>\nper\u00a0200mL<\/strong><\/td>\n<\/tr>\n
4<\/td>\nCaCl2\u00b72H2O\/\u00a0CaCl2<\/td>\n1.80g\/1.36g<\/td>\n3.6g\/2.72g<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n
Stock4<\/strong><\/td>\n\u8bd5\u5242<\/strong><\/td>\nper\u00a0100mL\u00a0<\/strong><\/td>\nper\u00a0200mL<\/strong><\/td>\n<\/tr>\n
5<\/td>\nNa2CO3<\/td>\n2.00g<\/td>\n4.00g<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n
Stock5<\/strong><\/td>\n\u5fae\u91cf<\/strong><\/td>\nper\u00a01000mL\u00a0<\/strong><\/td>\nper\u00a0500mL<\/strong><\/td>\n<\/tr>\n
<\/td>\nH3BO3<\/td>\n2.86g<\/td>\n1.43g<\/td>\n<\/tr>\n
<\/td>\nMnCl2\u00b74H2O<\/td>\n1.81g<\/td>\n0.91g<\/td>\n<\/tr>\n
<\/td>\nZnSO4\u00b77H2O<\/td>\n0.22g<\/td>\n0.11g<\/td>\n<\/tr>\n
<\/td>\nNa2MoO4\u00b72H2O<\/td>\n0.39g<\/td>\n0.20g<\/td>\n<\/tr>\n
<\/td>\nCuSO4\u00b75H2O<\/td>\n0.08g<\/td>\n0.04g<\/td>\n<\/tr>\n
<\/td>\nCo(NO3)2\u00a0\u00b76H2O\/CoCl2\u00a0\u00b76H2O<\/td>\n0.05g\/0.04g<\/td>\n0.025g\/0.02g<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u57f9\u517b\u57fa\u914d\u7f6e\uff1a<\/strong><\/p>\n\n\n\n\n\n\n\n\n
Medium<\/strong><\/td>\n\u00a0<\/strong><\/td>\nper\u00a01000mL<\/strong><\/td>\n<\/tr>\n
<\/td>\nStock\u00a0solution\u00a01<\/td>\n50.0mL<\/td>\n<\/tr>\n
<\/td>\nStock\u00a0solution\u00a02<\/td>\n2.0mL<\/td>\n<\/tr>\n
<\/td>\nStock\u00a0solution\u00a03<\/td>\n2.0mL<\/td>\n<\/tr>\n
<\/td>\nStock\u00a0solution\u00a04<\/td>\n1.0mL<\/td>\n<\/tr>\n
<\/td>\nStock\u00a0solution\u00a05<\/td>\n1.0mL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u7528NaOH<\/span>\u6216<\/span>HCl<\/span>\u8c03\u8282<\/span>pH<\/span>\u503c\u5230<\/span>7.1<\/span>\uff0c\u56fa\u4f53\u57f9\u517b\u57fa\u52a0<\/span>1.5%<\/span>\u7684\u743c\u8102\u7c89\u3002<\/span>12<\/span>1\u2103\uff0c0.1Mpa\uff0c\u706d\u83cc20min\u3002<\/p>\n

\u9002\u7528\u85fb\u79cd\uff1a<\/strong><\/p>\n

BG11\uff08<\/span>+N<\/span>\uff09\uff1a\u6d6e\u6e38\u98a4\u85fb\u3001\u83b1\u8335\u8863\u85fb<\/a>\u3001\u5c0f\u7403\u85fb<\/a>\u3001\u96c6\u80de\u85fb\u3001\u94dc\u7eff\u5fae\u56ca\u85fb<\/span>469<\/span>\u3001\u94dc\u7eff\u5fae\u56ca\u85fb<\/span>915<\/span>\u3001\u6c34\u534e\u675f\u4e1d\u85fb<\/span>\u3001\u6c34\u751f\u6240\u3001\u5bc6\u4e91\uff1b<\/p>\n

BG11\uff08<\/span>-N<\/span>\uff09\uff1a\u9c7c\u8165\u85fb<\/span>7120<\/span>\u3001\u53d1\u83dc\u85fb<\/span>\u3001\u6c34\u534e\u675f\u4e1d\u85fb<\/p>\n","protected":false},"excerpt":{"rendered":"

\u53c2\u8003\u6587\u732e\uff1aStanier,\u00a0R.Y.,\u00a0Kunisawa,\u00a0R.,\u00a0Mandel,\u00a0M.&\u00a0Cohen-Bazire,\u00a0G.1971.\u00a0Purification\u00a0and\u00a0properties\u00a0of\u00a0unicellular\u00a0blue-green\u00a0algae\u00a0(Order\u00a0Chroococcales).\u00a0Bacteriol.\u00a0Rev.\u00a035:\u00a0171-205. \u4e0a\u6d77\u5149\u8bed\u751f\u7269\u79d1\u6280\u6709\u9650\u516c\u53f8\u63d0\u4f9b\u914d\u7f6e […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[100],"tags":[108,371],"class_list":["post-973","post","type-post","status-publish","format-standard","hentry","category-medium","tag-bg11","tag-medium"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/973","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/comments?post=973"}],"version-history":[{"count":0,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/973\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/media?parent=973"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/categories?post=973"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/tags?post=973"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}