{"id":2942,"date":"2015-03-17T21:52:44","date_gmt":"2015-03-17T13:52:44","guid":{"rendered":"http:\/\/www.leadingtec.cn\/?p=2942"},"modified":"2015-03-17T21:52:44","modified_gmt":"2015-03-17T13:52:44","slug":"pro99-meida","status":"publish","type":"post","link":"https:\/\/www.leadingtec.cn\/pro99-meida.html","title":{"rendered":"Pro99\u57f9\u517b\u57fa\uff08Prochlorococcus \u539f\u7eff\u7403\u85fb\u57f9\u517b\u57fa\uff09"},"content":{"rendered":"\n\n\n\n\n\n
NaH2<\/sub>PO4<\/sub>\u00b7H2<\/sub>O solution (6.9 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
NH4<\/sub>Cl solution (42.8 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
Trace element solution<\/td>\n1 ml<\/td>\n<\/tr>\n
Oligotrophic seawater<\/td>\nto 1 L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Acid wash and dH2<\/sub>O rinse all glassware. Use oligotrophic (Sargasso Sea) seawater. Autoclave and cool before adding filter-sterilized nutrient stock solutions.<\/p>\n

Trace element solution:<\/b><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Na2<\/sub>EDTA\u00b72 H2<\/sub>O<\/td>\n0.436 g<\/td>\n<\/tr>\n
FeCl3<\/sub>\u00b76 H2<\/sub>O<\/td>\n0.316 g<\/td>\n<\/tr>\n
ZnSO4<\/sub>\u00b77 H2<\/sub>O solution (2.3 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
CoCl2<\/sub>\u00b76 H2<\/sub>O solution (1.19 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
MnCl2<\/sub>\u00b74 H2<\/sub>O solution (17.8 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
Na2<\/sub>MoO4<\/sub>\u00b72 H2<\/sub>O solution (0.73 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
Na2<\/sub>SeO3<\/sub> solution (1.29 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
NiSO4<\/sub>\u00b76 H2<\/sub>O solution (2.63 g\/L)<\/td>\n1 ml<\/td>\n<\/tr>\n
Distilled water<\/td>\nto 1 L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

NaH2PO4\u00b7H2O solution (6.9 g\/L) 1 ml NH4Cl solution (42.8 g\/L) 1 ml Trace element solution 1 ml Oligotrophic seawater to 1 L Acid wash and dH2O rinse all glassware. Use oligotrophic (Sargasso Sea) seaw […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[100],"tags":[371],"class_list":["post-2942","post","type-post","status-publish","format-standard","hentry","category-medium","tag-medium"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/2942","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/comments?post=2942"}],"version-history":[{"count":0,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/2942\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/media?parent=2942"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/categories?post=2942"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/tags?post=2942"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}