{"id":3587,"date":"2016-02-24T22:58:14","date_gmt":"2016-02-24T14:58:14","guid":{"rendered":"http:\/\/www.leadingtec.cn\/?p=3587"},"modified":"2016-03-05T11:32:18","modified_gmt":"2016-03-05T03:32:18","slug":"euglena-gracilis-medium","status":"publish","type":"post","link":"https:\/\/www.leadingtec.cn\/euglena-gracilis-medium.html","title":{"rendered":"Euglena gracilis\u57f9\u517b\u57fa\uff08EG medium\uff09"},"content":{"rendered":"

\u4e0a\u6d77\u5149\u8bed\u751f\u7269\u79d1\u6280\u6709\u9650\u516c\u53f8\u63d0\u4f9b\u7528\u4e8e\u88f8\u85fb\u3001\u773c\u866b\u57f9\u517bEuglena gracilis\u57f9\u517b\u57fa\uff08EG medium\uff09<\/span><\/p>\n

Freshwater\u00a0algae\u00a0and\u00a0protozoa<\/span>
\n Stock\u00a0\u00a0\u00a0(1)\u00a0\u00a0\u00a0\u00a0\u00a0CaCl2\u00a0stock\u00a0solution:\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0per\u00a0litre<\/span><\/strong>
\n CaCl2\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a01.0\u00a0g<\/span><\/p>\n

Medium\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0per\u00a0litre<\/span><\/strong>
\n Sodium\u00a0acetate\u00a0trihydrate\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a01.0\u00a0g<\/span><\/p>\n

“Lab-Lemco”\u00a0powder\u00a0(Oxoid\u00a0L29) \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 1.0\u00a0g<\/span><\/p>\n

*Tryptone\u00a0(Oxoid\u00a0L42)\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a02.0\u00a0g<\/span><\/p>\n

*Yeast\u00a0extract\u00a0(Oxoid\u00a0L21) \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a02.0\u00a0g<\/span><\/p>\n

CaCl2\u00a0stock\u00a0solution\u00a0(1)\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a010.0\u00a0ml<\/span><\/p>\n

Add\u00a0constituents\u00a0above\u00a0and\u00a0make\u00a0up\u00a0to\u00a01\u00a0litre\u00a0with\u00a0deionized\u00a0water.
\nFor\u00a0agar\u00a0add\u00a015\u00a0g\u00a0per\u00a0litre\u00a0*Bacteriological\u00a0Agar\u00a0(Oxoid\u00a0L11).
\nAutoclave\u00a0at\u00a015\u00a0psi\u00a0for\u00a015\u00a0minutes.<\/span><\/p>\n

Supply\u00a0*\u00a0Unipath\u00a0Ltd,\u00a0Wade\u00a0Road,\u00a0Basingstoke,\u00a0Hants\u00a0RG24 0PW,\u00a0UK<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u4e0a\u6d77\u5149\u8bed\u751f\u7269\u79d1\u6280\u6709\u9650\u516c\u53f8\u63d0\u4f9b\u7528\u4e8e\u88f8\u85fb\u3001\u773c\u866b\u57f9\u517bEuglena gracilis\u57f9\u517b\u57fa\uff08EG medium\uff09<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[100],"tags":[371],"class_list":["post-3587","post","type-post","status-publish","format-standard","hentry","category-medium","tag-medium"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/3587","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/comments?post=3587"}],"version-history":[{"count":0,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/3587\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/media?parent=3587"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/categories?post=3587"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/tags?post=3587"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}