{"id":5673,"date":"2018-01-22T10:29:55","date_gmt":"2018-01-22T02:29:55","guid":{"rendered":"http:\/\/www.leadingtec.cn\/?p=5673"},"modified":"2018-01-22T10:29:55","modified_gmt":"2018-01-22T02:29:55","slug":"cyanobacteria-poison","status":"publish","type":"post","link":"https:\/\/www.leadingtec.cn\/cyanobacteria-poison.html","title":{"rendered":"\u7701\u5343\u4eba\u97e9\u5175\u7537\u6559\u6388\u56e2\u961f\u5728\u6d77\u6d0b\u84dd\u85fb\u6bd2\u7d20\u7814\u7a76\u4e2d\u53d6\u5f97\u7a81\u7834"},"content":{"rendered":"<p><span style=\"font-size: 12pt;\">2018\u5e741\u670818\u65e5\uff0c\u7f8e\u56fd\u5316\u5b66\u5b66\u4f1a\uff08ACS\uff09\u6709\u673a\u5316\u5b66\u9886\u57df\u6743\u5a01\u671f\u520aOrganic Letters\u00a0(\u5f71\u54cd\u56e0\u5b506.6)\u00a0\u5728\u7ebf\u53d1\u8868\u4e86\u6d59\u6c5f\u7406\u5de5\u5927\u5b66\u751f\u547d\u79d1\u5b66\u5b66\u9662\u97e9\u5175\u7537\u6559\u6388\u56e2\u961f\u4e0e\u4e0a\u6d77\u4ea4\u5927\u533b\u5b66\u9662\u6797\u539a\u6587\u6559\u6388\u56e2\u961f\u5728\u6d77\u6d0b\u84dd\u85fb\u6bd2\u7d20Aplysiatoxins\u7684\u6d3b\u6027\u9776\u6807\u5408\u4f5c\u7814\u7a76\u4e2d\u53d6\u5f97\u7684\u91cd\u8981\u6210\u679c\u201cTwo Marine Cyanobacterial Aplysiatoxin Polyketides, Neo-debromoaplysiatoxin A and B, with K+ Channel Inhibition Activity\u201d\uff08DOI\uff1a10.1021\/acs.orglett.7b03672\uff09\u3002<\/span><\/p>\n<figure id=\"attachment_5674\" aria-describedby=\"caption-attachment-5674\" style=\"width: 633px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.leadingtec.cn\/wp-content\/uploads\/2018\/01\/1-4.png\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-5674\" src=\"https:\/\/www.leadingtec.cn\/wp-content\/uploads\/2018\/01\/1-4.png\" alt=\"Organic Letters\" width=\"633\" height=\"216\" srcset=\"https:\/\/www.leadingtec.cn\/wp-content\/uploads\/2018\/01\/1-4.png 633w, https:\/\/www.leadingtec.cn\/wp-content\/uploads\/2018\/01\/1-4-600x205.png 600w, https:\/\/www.leadingtec.cn\/wp-content\/uploads\/2018\/01\/1-4-300x102.png 300w\" sizes=\"auto, (max-width: 633px) 100vw, 633px\" \/><\/a><figcaption id=\"caption-attachment-5674\" class=\"wp-caption-text\"><\/span> <span style=\"font-size: 12pt;\">Organic Letters<\/span><\/figcaption><\/figure>\n<p><span style=\"font-size: 12pt;\">Aplysiatoxins\u4e00\u76f4\u4ee5\u6765\u88ab\u8ba4\u4e3a\u662f\u4e00\u7c7b\u6709\u4e89\u8bae\u7684\u6d77\u6d0b\u751f\u7269\u6bd2\u7d20\uff0c\u7814\u7a76\u663e\u793a\u5176\u5177\u6709PKC\u6fc0\u9176\u6fc0\u6d3b\u4f5c\u7528\uff0c\u4ece\u800c\u4ea7\u751f\u591a\u79cd\u751f\u7269\u6d3b\u6027\uff0c\u4fc3\u7624\u3001\u6297\u80bf\u7624\u53ca\u6297\u75c5\u6bd2\u7b49\u3002\u672c\u7814\u7a76\u5728\u91c7\u81ea\u4e2d\u56fd\u5357\u6d77\u7684\u84dd\u85fbLyngbya sp.\u4ee3\u8c22\u7269\u4e2d\u9996\u6b21\u53d1\u73b0\u4e86\u4e24\u4e2a\u7ed3\u6784\u65b0\u9896\u7684aplysiatoxins\u7c7b\u4f3c\u7269\uff0cNeo-debromoaplysiatoxin A and B\uff08\u5316\u5408\u72691-2\uff09\u3002 \u5e76\u8fd0\u7528\u73b0\u4ee3\u6ce2\u8c31\u5b66\u624b\u6bb5\uff0c\u7ed3\u5408X-ray\u5355\u6676\u884d\u5c04\u548cECD\u5206\u5b50\u8ba1\u7b97\u786e\u5b9a\u5176\u5e73\u9762\u53ca\u7acb\u4f53\u7ed3\u6784\u3002\u7814\u7a76\u4eba\u5458\u4f7f\u7528\u94a0\u79bb\u5b50\u901a\u9053\uff08Nav1.5\uff0cNav1.7\uff0cNav1.8\uff09\u548c\u94be\u79bb\u5b50\u901a\u9053\uff08hERG\uff0cKv1.5\uff0cKir2.1\uff09\u5bf9\u5316\u5408\u72691\u548c2\u8fdb\u884c\u4e86\u6d3b\u6027\u7b5b\u9009\uff0c\u7ed3\u679c\u663e\u793a\u5316\u5408\u72691\u548c2\u53ea\u5bf9\u94be\u79bb\u5b50\u901a\u9053Kv1.5\u6709\u5f3a\u70c8\u6291\u5236\u4f5c\u7528\uff08IC50\u503c\u5206\u522b\u4e3a6.94\u00b10.26\u00a0\u03bcM\u548c0.30\u00b10.05 \u03bcM\uff09\u3002<\/span><\/p>\n<figure id=\"attachment_5677\" aria-describedby=\"caption-attachment-5677\" style=\"width: 443px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.leadingtec.cn\/wp-content\/uploads\/2018\/01\/4-2.png\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-5677\" src=\"https:\/\/www.leadingtec.cn\/wp-content\/uploads\/2018\/01\/4-2.png\" alt=\"Dose\u2212response study of neo-debromoaplysiatoxin A (1) and B (2) with Kv1.5 expression in CHO cells\" width=\"443\" height=\"223\" srcset=\"https:\/\/www.leadingtec.cn\/wp-content\/uploads\/2018\/01\/4-2.png 443w, https:\/\/www.leadingtec.cn\/wp-content\/uploads\/2018\/01\/4-2-300x151.png 300w\" sizes=\"auto, (max-width: 443px) 100vw, 443px\" \/><\/a><figcaption id=\"caption-attachment-5677\" class=\"wp-caption-text\"><\/span> <span style=\"font-size: 12pt;\">Dose\u2212response study of neo-debromoaplysiatoxin A (1) and B (2) with Kv1.5 expression in CHO cells<\/span><\/figcaption><\/figure>\n<p><span style=\"font-size: 12pt;\">\u5728PKC\u6fc0\u9176\u6fc0\u6d3b\u5b9e\u9a8c\u4e2d\u53d1\u73b0\uff0c\u5316\u5408\u72691\u548c\u5df2\u77e5\u5316\u5408\u7269debromoaplysiatoxin\u90fd\u80fd\u591f\u4fc3\u4f7fPKC\u6fc0\u9176\u78f7\u9178\u5316\uff0c\u800c\u5316\u5408\u72692\u5373\u4f7f\u572810 \u03bcM\u7684\u6d53\u5ea6\u4e0b\u5bf9PKC\u03b4\u5e76\u6ca1\u6709\u663e\u793a\u4efb\u4f55\u6fc0\u6d3b\u4f5c\u7528\u3002\u4ece\u7ed3\u679c\u63a8\u6d4b\uff0c\u5316\u5408\u72692\u8ddf\u4ee5\u5f80aplysiatoxins\u4f5c\u4e3aPKC\u6fc0\u9176\u6fc0\u6d3b\u5242\u7684\u8c03\u63a7\u673a\u5236\u4e0d\u4e00\u6837\uff0c\u662f\u4e00\u79cd\u65b0\u578b\u94be\u79bb\u5b50\u901a\u9053\u6291\u5236\u5242\uff0c\u4e3a\u4eca\u540e\u7814\u7a76aplysiatoxins\u4f5c\u4e3aPKC\u6fc0\u9176\u6fc0\u6d3b\u5242\u6765\u8c03\u63a7\u4e0b\u6e38\u4fe1\u53f7\u901a\u8def\u63d0\u4f9b\u4e86\u4e0d\u4e00\u6837\u7684\u601d\u8def\u3002Kv1.5\u94be\u79bb\u5b50\u901a\u9053\u4ec5\u5728\u4eba\u5fc3\u623f\u808c\u4e2d\u8868\u8fbe\uff0c\u662f\u4e00\u79cd\u623f\u98a4\u6cbb\u7597\u7684\u65b0\u9776\u70b9\uff0c\u7279\u5f02\u6027Kv1.5\u901a\u9053\u963b\u6ede\u5242\u5bf9\u5fc3\u623f\u5177\u6709\u9ad8\u5ea6\u9009\u62e9\u6027\uff0c\u4e0d\u5f15\u53d1\u5ba4\u6027\u5fc3\u5f8b\u5931\u5e38\uff0c\u6781\u6709\u53ef\u80fd\u6210\u4e3a\u672a\u6765\u5fc3\u623f\u98a4\u52a8\u6cbb\u7597\u7684\u65b0\u578b\u4e3b\u5bfc\u836f\u7269\u4e4b\u4e00\u3002<\/span><\/p>\n<figure id=\"attachment_5676\" aria-describedby=\"caption-attachment-5676\" style=\"width: 443px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.leadingtec.cn\/wp-content\/uploads\/2018\/01\/3-4.png\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-5676\" src=\"https:\/\/www.leadingtec.cn\/wp-content\/uploads\/2018\/01\/3-4.png\" alt=\"Effect of debromoaplysiatoxin, compounds 1\u00a0and\u00a02\u00a0on phosphor-PKC\u03b4 expression in HepG2 cells. (A), (C), (E)\u00a0Cells were treated with indicated concentration (0.5 \u00b5M, 5 \u00b5M, 10 \u00b5M) of\u00a0debromoaplysiatoxin, compounds 1\u00a0and 2, PMA (0.1 \u00b5M) for 1 h and the expression of\u00a0p-PKC\u03b4 protein was determined to use cell lysates by western blotting. The pictures of (B),\u00a0(D) and (F) are the corresponding statistical data results.\" width=\"443\" height=\"431\" srcset=\"https:\/\/www.leadingtec.cn\/wp-content\/uploads\/2018\/01\/3-4.png 443w, https:\/\/www.leadingtec.cn\/wp-content\/uploads\/2018\/01\/3-4-300x292.png 300w\" sizes=\"auto, (max-width: 443px) 100vw, 443px\" \/><\/a><figcaption id=\"caption-attachment-5676\" class=\"wp-caption-text\"><\/span> <span style=\"font-size: 12pt;\">Effect of debromoaplysiatoxin, compounds 1\u00a0and\u00a02\u00a0on phosphor-PKC\u03b4 expression in HepG2 cells. (A), (C), (E)\u00a0Cells were treated with indicated concentration (0.5 \u00b5M, 5 \u00b5M, 10 \u00b5M) of\u00a0debromoaplysiatoxin, compounds 1\u00a0and 2, PMA (0.1 \u00b5M) for 1 h and the expression of\u00a0p-PKC\u03b4 protein was determined to use cell lysates by western blotting. The pictures of (B),\u00a0(D) and (F) are the corresponding statistical data results.<\/span><\/figcaption><\/figure>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: 12pt;\">\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/span><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7701\u5343\u4eba\u97e9\u5175\u7537\u6559\u6388\u56e2\u961f\u5728\u6d77\u6d0b\u84dd\u85fb\u6bd2\u7d20\u7814\u7a76\u4e2d\u53d6\u5f97\u7a81\u7834<\/p>\n","protected":false},"author":70,"featured_media":5677,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[65],"class_list":["post-5673","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-info","tag-65"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/5673","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/users\/70"}],"replies":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/comments?post=5673"}],"version-history":[{"count":0,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/5673\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/media\/5677"}],"wp:attachment":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/media?parent=5673"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/categories?post=5673"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/tags?post=5673"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}