{"id":7867,"date":"2021-05-11T17:14:58","date_gmt":"2021-05-11T09:14:58","guid":{"rendered":"http:\/\/www.leadingtec.cn\/?p=7867"},"modified":"2021-05-11T17:25:17","modified_gmt":"2021-05-11T09:25:17","slug":"walnes-medium","status":"publish","type":"post","link":"https:\/\/www.leadingtec.cn\/walnes-medium.html","title":{"rendered":"Walne’s \u57f9\u517b\u57fa (Walne’s medium)"},"content":{"rendered":"

Walnes \u57f9\u517b\u57fa (Walnes medium)\u662f\u4e00\u79cd\u56fd\u5916\u5e38\u7528\u7684\u6d77\u6c34\u85fb\u7c7b\u57f9\u517b\u57fa\uff0c\u5149\u8bed\u751f\u7269\u63d0\u4f9b\u5546\u54c1\u88c5\u57f9\u517b\u57fa\uff0c\u5ba2\u6237\u4e5f\u53ef\u4ee5\u6839\u636e\u5982\u4e0b\u914d\u65b9\u914d\u7f6e\u3002<\/span><\/h1>\n

Walne\u2019s Medium Composition<\/span><\/h1>\n

From https:\/\/biocyclopedia.com\/index\/algae\/algal_culturing\/walnes_medium_composition.php<\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Reagents<\/span><\/th>\nPer Liter Seawater<\/span><\/th>\n<\/tr>\n<\/thead>\n
Solution A<\/span><\/td>\n1.0 ml<\/span><\/td>\n<\/tr>\n
Solution C<\/span><\/td>\n0.1 ml<\/span><\/td>\n<\/tr>\n
Solution D (to add for diatoms)<\/span><\/td>\n2.0 ml<\/span><\/td>\n<\/tr>\n
Solution A<\/strong><\/span><\/td>\n<\/tr>\n
FeCl3<\/sub>\u00a0* 6H2<\/sub>O<\/span><\/td>\n1.3 g<\/span><\/td>\n<\/tr>\n
MnCl2<\/sub>\u00a0* 4H2<\/sub>O<\/span><\/td>\n0.4 g<\/span><\/td>\n<\/tr>\n
H3<\/sub>BO3<\/sub><\/span><\/td>\n33.6 g<\/span><\/td>\n<\/tr>\n
Na2<\/sub>EDTA<\/span><\/td>\n45.0 g<\/span><\/td>\n<\/tr>\n
NaH2<\/sub>PO4<\/sub>\u00a0* 2H2<\/sub>O<\/span><\/td>\n20.0 g<\/span><\/td>\n<\/tr>\n
NaNO3<\/sub><\/span><\/td>\n100.0 g<\/span><\/td>\n<\/tr>\n
Solution B<\/span><\/td>\n1 ml<\/span><\/td>\n<\/tr>\n
Solution B per 100ml<\/strong><\/span><\/td>\n<\/tr>\n
ZnCl2<\/sub><\/span><\/td>\n2.1 g<\/span><\/td>\n<\/tr>\n
CoCl2<\/sub>\u00a0* 6H2<\/sub>O<\/span><\/td>\n2.0 g<\/span><\/td>\n<\/tr>\n
(NH4<\/sub>)6<\/sub>Mo7<\/sub>O24<\/sub>\u00a0* 4H2<\/sub>O<\/span><\/td>\n0.9 g<\/span><\/td>\n<\/tr>\n
CuSO4<\/sub>\u00a0* 5H2<\/sub>O<\/span><\/td>\n2.0 g<\/span><\/td>\n<\/tr>\n
Concentrated HCl<\/span><\/td>\n10 ml<\/span><\/td>\n<\/tr>\n
Solution C per 200ml<\/strong><\/span><\/td>\n<\/tr>\n
Thiamine HCl (Vitamin B1<\/sub>)<\/span><\/td>\n0.2 g<\/span><\/td>\n<\/tr>\n
Cyanocobalamin (Vitamin B12<\/sub>)<\/span><\/td>\n10 mg<\/span><\/td>\n<\/tr>\n
Solution D (per liter)<\/strong><\/span><\/td>\n<\/tr>\n
Na2<\/sub>SiO3<\/sub>\u00a0* 5H2<\/sub>O<\/span><\/td>\n40.0 g<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

Walnes \u57f9\u517b\u57fa (Walnes medium)\u662f\u4e00\u79cd\u56fd\u5916\u5e38\u7528\u7684\u6d77\u6c34\u85fb\u7c7b\u57f9\u517b\u57fa\uff0c\u5149\u8bed\u751f\u7269\u63d0\u4f9b\u5546\u54c1\u88c5\u57f9\u517b\u57fa\uff0c\u5ba2\u6237\u4e5f\u53ef\u4ee5\u6839\u636e\u5982\u4e0b\u914d\u65b9\u914d\u7f6e\u3002 Walne\u2019s Medium Composition From https:\/\/biocyclopedia.com\/index\/algae\/algal_culturing\/walnes_medium_composition.php Reagents Per L […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[100],"tags":[371],"class_list":["post-7867","post","type-post","status-publish","format-standard","hentry","category-medium","tag-medium"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/7867","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/comments?post=7867"}],"version-history":[{"count":0,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/posts\/7867\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/media?parent=7867"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/categories?post=7867"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.leadingtec.cn\/wp-json\/wp\/v2\/tags?post=7867"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}