{"id":9015,"date":"2025-02-18T09:53:59","date_gmt":"2025-02-18T01:53:59","guid":{"rendered":"https:\/\/www.leadingtec.cn\/?p=9015"},"modified":"2025-02-18T09:54:00","modified_gmt":"2025-02-18T01:54:00","slug":"%e5%be%ae%e8%97%bb%e5%af%86%e5%ba%a6%e4%b8%8eod%e5%80%bc%e7%9a%84%e7%a7%98%e5%af%86-%e5%a6%82%e4%bd%95%e5%bf%ab%e9%80%9f%e6%a3%80%e6%b5%8b%e5%be%ae%e8%97%bb%e7%94%9f%e9%95%bf%ef%bc%9f","status":"publish","type":"post","link":"https:\/\/www.leadingtec.cn\/%e5%be%ae%e8%97%bb%e5%af%86%e5%ba%a6%e4%b8%8eod%e5%80%bc%e7%9a%84%e7%a7%98%e5%af%86-%e5%a6%82%e4%bd%95%e5%bf%ab%e9%80%9f%e6%a3%80%e6%b5%8b%e5%be%ae%e8%97%bb%e7%94%9f%e9%95%bf%ef%bc%9f.html","title":{"rendered":"\u5fae\u85fb\u5bc6\u5ea6\u4e0eOD\u503c\u7684\u79d8\u5bc6\u2014\u2014\u5982\u4f55\u5feb\u901f\u68c0\u6d4b\u5fae\u85fb\u751f\u957f\uff1f"},"content":{"rendered":"

1. \u5f15\u8a00<\/strong><\/h3>

\u5927\u5bb6\u597d\uff01\u4eca\u5929\u6211\u4eec\u6765\u804a\u4e00\u804a\u5fae\u85fb\u57f9\u517b\u4e2d\u7684\u4e00\u4e2a\u6709\u8da3\u8bdd\u9898\u2014\u2014\u5982\u4f55\u901a\u8fc7OD\u503c\u5feb\u901f\u68c0\u6d4b\u5fae\u85fb\u5bc6\u5ea6<\/strong>\u3002
\u5fae\u85fb\u662f\u4e00\u79cd\u5fae\u5c0f\u7684\u6c34\u751f\u690d\u7269\uff0c\u5b83\u4eec\u4e0d\u4ec5\u53ef\u4ee5\u7528\u6765\u751f\u4ea7\u751f\u7269\u71c3\u6599\uff0c\u8fd8\u80fd\u5236\u4f5c\u98df\u54c1\u3001\u836f\u54c1\uff0c\u751a\u81f3\u51c0\u5316\u73af\u5883\u3002
\u5728\u5fae\u85fb\u57f9\u517b\u4e2d\uff0c\u4e86\u89e3\u5b83\u4eec\u7684\u751f\u957f\u72b6\u6001\u975e\u5e38\u91cd\u8981\u3002\u4f20\u7edf\u65b9\u6cd5\u867d\u7136\u51c6\u786e\uff0c\u4f46\u8017\u65f6\u957f\u3001\u6210\u672c\u9ad8\u3002
\u800c\u4eca\u5929\u6211\u4eec\u8981\u4ecb\u7ecd\u7684OD\u6cd5\uff08\u5149\u5bc6\u5ea6\u6cd5\uff09<\/strong>\uff0c\u5219\u662f\u4e00\u79cd\u5feb\u901f\u3001\u7b80\u4fbf\u3001\u4f4e\u6210\u672c\u7684\u9009\u62e9\u3002
\u63a5\u4e0b\u6765\uff0c\u8ba9\u6211\u4eec\u4e00\u8d77\u63a2\u7d22OD\u503c\u4e0e\u5fae\u85fb\u5bc6\u5ea6\u7684\u5173\u7cfb\u5427\uff01<\/p>

2. \u4ec0\u4e48\u662fOD\u503c\uff1f<\/strong><\/h4>

OD\u503c\uff0c\u5168\u79f0\u5149\u5bc6\u5ea6\uff08Optical Density\uff09<\/strong>\uff0c\u662f\u7528\u6765\u8861\u91cf\u6db2\u4f53\u4e2d\u5fae\u85fb\u6d53\u5ea6\u7684\u6307\u6807\u3002
\u5b83\u7684\u539f\u7406\u5f88\u7b80\u5355\uff1a\u5f53\u5149\u7a7f\u8fc7\u5fae\u85fb\u6db2\u65f6\uff0c\u5fae\u85fb\u7ec6\u80de\u4f1a\u5438\u6536\u4e00\u90e8\u5206\u5149\uff0c\u5438\u6536\u5f97\u8d8a\u591a\uff0cOD\u503c\u5c31\u8d8a\u9ad8\u3002
\u901a\u5e38\uff0c\u6211\u4eec\u4f7f\u7528\u5206\u5149\u5149\u5ea6\u8ba1\u5728680nm\u6ce2\u957f\u4e0b\u6d4b\u5b9aOD\u503c\uff0c\u56e0\u4e3a\u8fd9\u662f\u53f6\u7eff\u7d20a\u7684\u5438\u6536\u5cf0\u3002<\/p>

3. \u5982\u4f55\u5efa\u7acbOD\u503c\u4e0e\u5fae\u85fb\u5bc6\u5ea6\u7684\u5173\u7cfb\uff1f<\/strong><\/h4>

\u8981\u5efa\u7acbOD\u503c\u4e0e\u5fae\u85fb\u5bc6\u5ea6\u7684\u5173\u7cfb\uff0c\u6211\u4eec\u9700\u8981\u505a\u4e00\u4e2a\u5c0f\u5b9e\u9a8c\uff0c\u5206\u4e3a\u4ee5\u4e0b\u51e0\u6b65\uff1a<\/p>

\u7b2c\u4e00\u6b65\uff1a\u57f9\u517b\u5fae\u85fb\u5e76\u5236\u5907\u7a00\u91ca\u6db2<\/strong>
\u9996\u5148\uff0c\u6211\u4eec\u9700\u8981\u5728\u9002\u5b9c\u6761\u4ef6\u4e0b\u57f9\u517b\u5fae\u85fb\uff0c\u786e\u4fdd\u5b83\u4eec\u5904\u4e8e\u5feb\u901f\u751f\u957f\u671f\u3002
\u7136\u540e\uff0c\u7528\u65e0\u83cc\u57f9\u517b\u57fa\u5bf9\u5fae\u85fb\u6db2\u8fdb\u884c\u68af\u5ea6\u7a00\u91ca\uff0c\u6bd4\u59821:1\u30011:2\u30011:4\u7b49\uff0c\u83b7\u5f97\u4e0d\u540c\u5bc6\u5ea6\u7684\u6837\u54c1\u3002<\/p>

\u7b2c\u4e8c\u6b65\uff1a\u6d4b\u5b9aOD\u503c<\/strong>
\u5c06\u7a00\u91ca\u540e\u7684\u6837\u54c1\u52a0\u5165\u6bd4\u8272\u76bf\uff0c\u4f7f\u7528\u5206\u5149\u5149\u5ea6\u8ba1\u5728680nm\u6ce2\u957f\u4e0b\u6d4b\u5b9aOD\u503c\u3002
\u6ce8\u610f\uff1a\u6bcf\u6b21\u6d4b\u5b9a\u524d\u8981\u7528\u65e0\u83cc\u57f9\u517b\u57fa\u4f5c\u4e3a\u7a7a\u767d\u5bf9\u7167\uff0c\u786e\u4fdd\u7ed3\u679c\u51c6\u786e\u3002<\/p>

\u7b2c\u4e09\u6b65\uff1a\u7cbe\u786e\u8ba1\u6570\u5fae\u85fb\u5bc6\u5ea6<\/strong>
\u4f7f\u7528\u7ec6\u80de\u8ba1\u6570\u677f\u6216\u81ea\u52a8\u7ec6\u80de\u8ba1\u6570\u5668\uff0c\u5bf9\u6bcf\u4e2a\u7a00\u91ca\u6837\u54c1\u7684\u5fae\u85fb\u5bc6\u5ea6\u8fdb\u884c\u7cbe\u786e\u8ba1\u6570\uff0c\u5355\u4f4d\u662fcells\/mL\u3002<\/p>

\u7b2c\u56db\u6b65\uff1a\u7ed8\u5236\u6807\u51c6\u66f2\u7ebf<\/strong>
\u4ee5OD\u503c\u4e3a\u6a2a\u8f74\uff0c\u5fae\u85fb\u5bc6\u5ea6\u4e3a\u7eb5\u8f74\uff0c\u7ed8\u5236\u6563\u70b9\u56fe\uff0c\u5e76\u901a\u8fc7\u7ebf\u6027\u56de\u5f52\u62df\u5408\u51fa\u6807\u51c6\u66f2\u7ebf\u65b9\u7a0b\uff0c\u6bd4\u5982 ( y = kx + b )\u3002<\/p>

4. \u5b9e\u9a8c\u4e2d\u7684\u5c0f\u8d34\u58eb<\/strong><\/h4>

\u5728\u5b9e\u9a8c\u8fc7\u7a0b\u4e2d\uff0c\u6709\u4e00\u4e9b\u5c0f\u7ec6\u8282\u9700\u8981\u6ce8\u610f\uff1a<\/p>

1. \u6837\u54c1\u5904\u7406<\/strong><\/p>

  • \u6d4b\u5b9a\u524d\u8981\u5145\u5206\u6df7\u5300\u6837\u54c1\uff0c\u907f\u514d\u5fae\u85fb\u6c89\u6dc0\u5bfc\u81f4\u8bef\u5dee\u3002<\/li>\n\n
  • \u7a00\u91ca\u540e\u7684OD\u503c\u5e94\u63a7\u5236\u57280.1-0.8\u4e4b\u95f4\uff0c\u8fd9\u662f\u5206\u5149\u5149\u5ea6\u8ba1\u7684\u6700\u4f73\u7ebf\u6027\u8303\u56f4\u3002<\/li>\n\n
  • \u6bd4\u8272\u76bf\u4f7f\u7528\u524d\u540e\u8981\u7528\u84b8\u998f\u6c34\u6e05\u6d17\uff0c\u907f\u514d\u6b8b\u7559\u5f71\u54cd\u7ed3\u679c\u3002<\/li><\/ul>

    2. \u6d4b\u5b9a\u6761\u4ef6<\/strong><\/p>

    • \u6ce2\u957f\u901a\u5e38\u9009\u62e9680nm\uff0c\u56e0\u4e3a\u8fd9\u662f\u53f6\u7eff\u7d20a\u7684\u5438\u6536\u5cf0\u3002<\/li>\n\n
    • \u6bcf\u6b21\u6d4b\u5b9a\u90fd\u8981\u7528\u65e0\u83cc\u57f9\u517b\u57fa\u4f5c\u4e3a\u7a7a\u767d\u5bf9\u7167\u3002<\/li>\n\n
    • \u5206\u5149\u5149\u5ea6\u8ba1\u8981\u5b9a\u671f\u6821\u51c6\uff0c\u786e\u4fdd\u6570\u636e\u51c6\u786e\u3002<\/li><\/ul>

      3. \u7ec6\u80de\u8ba1\u6570<\/strong><\/p>

      • \u4f7f\u7528\u8840\u7403\u8ba1\u6570\u677f\u65f6\uff0c\u9075\u5faa\u201c\u6570\u4e0a\u4e0d\u6570\u4e0b\uff0c\u6570\u5de6\u4e0d\u6570\u53f3\u201d\u7684\u539f\u5219\uff0c\u907f\u514d\u91cd\u590d\u8ba1\u6570\u3002<\/li>\n\n
      • \u6bcf\u4e2a\u6837\u54c1\u81f3\u5c11\u8ba1\u65703\u6b21\uff0c\u53d6\u5e73\u5747\u503c\u4ee5\u51cf\u5c11\u8bef\u5dee\u3002<\/li>\n\n
      • \u6ce8\u610f\u5fae\u85fb\u5f62\u6001\u53d8\u5316\uff0c\u6bd4\u5982\u805a\u96c6\u6216\u7834\u88c2\uff0c\u786e\u4fdd\u8ba1\u6570\u7ed3\u679c\u51c6\u786e\u3002<\/li><\/ul>

        4. \u6807\u51c6\u66f2\u7ebf\u7684\u9002\u7528\u6027<\/strong><\/p>

        • \u4e0d\u540c\u85fb\u79cd\u7684OD-\u5bc6\u5ea6\u5173\u7cfb\u53ef\u80fd\u4e0d\u540c\uff0c\u9700\u8981\u5206\u522b\u5efa\u7acb\u6807\u51c6\u66f2\u7ebf\u3002<\/li>\n\n
        • \u57f9\u517b\u6761\u4ef6\uff08\u5982\u5149\u7167\u3001\u6e29\u5ea6\uff09\u53d8\u5316\u53ef\u80fd\u5f71\u54cd\u7ed3\u679c\uff0c\u9700\u91cd\u65b0\u9a8c\u8bc1\u3002<\/li>\n\n
        • \u6307\u6570\u751f\u957f\u671f\u7684\u5fae\u85fbOD-\u5bc6\u5ea6\u5173\u7cfb\u66f4\u7a33\u5b9a\uff0c\u8870\u8001\u671f\u53ef\u80fd\u504f\u79bb\u7ebf\u6027\u3002<\/li><\/ul>

          5. \u6807\u51c6\u66f2\u7ebf\u7684\u9a8c\u8bc1\u4e0e\u5e94\u7528<\/strong><\/h4>

          \u5efa\u7acb\u6807\u51c6\u66f2\u7ebf\u540e\uff0c\u6211\u4eec\u9700\u8981\u5bf9\u5176\u8fdb\u884c\u9a8c\u8bc1\u3002
          \u901a\u8fc7\u91cd\u590d\u5b9e\u9a8c\u548c\u8bef\u5dee\u5206\u6790\uff0c\u786e\u4fdd\u7ebf\u6027\u56de\u5f52\u7684\u76f8\u5173\u7cfb\u6570\uff08R\u00b2\uff09\u8fbe\u52300.98\u4ee5\u4e0a\uff0c\u8868\u660e\u7ebf\u6027\u5173\u7cfb\u826f\u597d\u3002
          \u9a8c\u8bc1\u901a\u8fc7\u540e\uff0c\u6807\u51c6\u66f2\u7ebf\u5c31\u53ef\u4ee5\u5e7f\u6cdb\u5e94\u7528\u4e8e\u5feb\u901f\u68c0\u6d4b\u548c\u52a8\u6001\u76d1\u6d4b\u4e2d\u3002
          \u6bd4\u5982\uff0c\u5728\u540e\u7eed\u5b9e\u9a8c\u4e2d\uff0c\u53ea\u9700\u6d4b\u5b9a\u6837\u54c1\u7684OD\u503c\uff0c\u5c31\u80fd\u901a\u8fc7\u6807\u51c6\u66f2\u7ebf\u65b9\u7a0b\u5feb\u901f\u8ba1\u7b97\u51fa\u5fae\u85fb\u5bc6\u5ea6\u3002
          \u6211\u4eec\u8fd8\u53ef\u4ee5\u5b9a\u671f\u6d4b\u5b9aOD\u503c\uff0c\u7ed8\u5236\u751f\u957f\u66f2\u7ebf\uff0c\u8bc4\u4f30\u5fae\u85fb\u7684\u751f\u957f\u72b6\u6001\u3002<\/p>

          6. \u5b9e\u9645\u6848\u4f8b\uff1a\u5c0f\u7403\u85fb\u7684OD-\u5bc6\u5ea6\u6807\u51c6\u66f2\u7ebf<\/strong><\/h4>

          \u8ba9\u6211\u4eec\u901a\u8fc7\u4e00\u4e2a\u5b9e\u9645\u6848\u4f8b\uff0c\u5e2e\u52a9\u5927\u5bb6\u66f4\u597d\u5730\u7406\u89e3\u8fd9\u4e00\u8fc7\u7a0b\u3002
          \u6211\u4eec\u4ee5\u5c0f\u7403\u85fb\u4e3a\u4f8b\uff0c\u5efa\u7acb\u5176OD-\u5bc6\u5ea6\u6807\u51c6\u66f2\u7ebf\u3002<\/p>

          \u6570\u636e\u5982\u4e0b<\/strong>\uff1a<\/p>

          OD680<\/th>\u5fae\u85fb\u5bc6\u5ea6\uff08cells\/mL\uff09<\/th><\/tr><\/thead>
          0.1<\/td>1.0\u00d710\u2076<\/td><\/tr>
          0.2<\/td>2.1\u00d710\u2076<\/td><\/tr>
          0.4<\/td>4.0\u00d710\u2076<\/td><\/tr>
          0.6<\/td>6.2\u00d710\u2076<\/td><\/tr>
          0.8<\/td>8.1\u00d710\u2076<\/td><\/tr><\/tbody><\/table><\/figure>

          \u62df\u5408\u7684\u7ebf\u6027\u65b9\u7a0b<\/strong>\uff1a
          ( y = 1.02 \\times 10^7 x + 1.5 \\times 10^5 )
          \u76f8\u5173\u7cfb\u6570R\u00b2=0.995\uff0c\u8868\u660e\u7ebf\u6027\u5173\u7cfb\u975e\u5e38\u597d\u3002<\/p>

          \u5e94\u7528\u793a\u4f8b<\/strong>\uff1a
          \u5982\u679c\u67d0\u6837\u54c1\u7684OD680\u503c\u4e3a0.5\uff0c\u90a3\u4e48\u5176\u5fae\u85fb\u5bc6\u5ea6\u53ef\u4ee5\u901a\u8fc7\u65b9\u7a0b\u8ba1\u7b97\uff1a
          ( y = 1.02 \\times 10^7 \\times 0.5 + 1.5 \\times 10^5 = 5.25 \\times 10^6 \\, \\text{cells\/mL} )<\/p>

          7. \u603b\u7ed3\u4e0e\u5c55\u671b<\/strong><\/h4>

          \u901a\u8fc7\u5efa\u7acbOD-\u5bc6\u5ea6\u7ebf\u6027\u5173\u7cfb\uff0c\u6211\u4eec\u53ef\u4ee5\u5b9e\u73b0\u5fae\u85fb\u5bc6\u5ea6\u7684\u5feb\u901f\u68c0\u6d4b\uff0c\u663e\u8457\u63d0\u5347\u57f9\u517b\u6548\u7387\uff0c\u964d\u4f4e\u6210\u672c\u3002
          \u8fd9\u9879\u6280\u672f\u7684\u6838\u5fc3\u4ef7\u503c\u5728\u4e8e\u5b83\u7684\u5feb\u901f\u3001\u51c6\u786e\u548c\u4f4e\u6210\u672c<\/strong>\uff0c\u975e\u5e38\u9002\u5408\u5927\u89c4\u6a21\u751f\u4ea7\u4e2d\u7684\u5e94\u7528\u3002
          \u672a\u6765\uff0c\u968f\u7740\u81ea\u52a8\u5316\u68c0\u6d4b\u548c\u667a\u80fd\u5316\u5206\u6790\u6280\u672f\u7684\u53d1\u5c55\uff0c\u8fd9\u9879\u6280\u672f\u5c06\u53d8\u5f97\u66f4\u52a0\u9ad8\u6548\u548c\u4fbf\u6377\u3002<\/p>

          8. \u5c0f\u95ee\u9898<\/strong><\/h4>

          \u6700\u540e\uff0c\u7559\u4e00\u4e2a\u5c0f\u95ee\u9898\u7ed9\u5927\u5bb6\u601d\u8003\uff1a
          \u5982\u679c\u4f60\u8981\u57f9\u517b\u4e00\u79cd\u65b0\u7684\u5fae\u85fb\uff0c\u5982\u4f55\u8bbe\u8ba1\u5b9e\u9a8c\u6765\u5efa\u7acb\u5b83\u7684OD-\u5bc6\u5ea6\u6807\u51c6\u66f2\u7ebf\u5462\uff1f
          \u6b22\u8fce\u5728\u8bc4\u8bba\u533a\u5206\u4eab\u4f60\u7684\u60f3\u6cd5\uff01<\/p>","protected":false},"excerpt":{"rendered":"

          OD\u503c\uff0c\u5168\u79f0\u5149\u5bc6\u5ea6\uff08Optical Density\uff09\uff0c\u662f\u7528\u6765\u8861\u91cf\u6db2\u4f53\u4e2d\u5fae\u85fb\u6d53\u5ea6\u7684\u6307\u6807\u3002
          \n\u5b83\u7684\u539f\u7406\u5f88\u7b80\u5355\uff1a\u5f53\u5149\u7a7f\u8fc7\u5fae\u85fb\u6db2\u65f6\uff0c\u5fae\u85fb\u7ec6\u80de\u4f1a\u5438\u6536\u4e00\u90e8\u5206\u5149\uff0c\u5438\u6536\u5f97\u8d8a\u591a\uff0cOD\u503c\u5c31\u8d8a\u9ad8\u3002
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