\u4e00\u3001\u6b63\u5e38\u751f\u957f\u72b6\u6001\u4e0b\u7684\u989c\u8272\u7279\u5f81<\/p>
\u5065\u5eb7\u751f\u957f\u671f\uff1a<\/p>
\u989c\u8272\uff1a\u6df1\u7ea2\u8272\u3001\u7d2b\u7ea2\u8272\u6216\u7c89\u7ea2\u8272\uff08\u56e0\u83cc\u682a\u548c\u5149\u7167\u6761\u4ef6\u7565\u6709\u5dee\u5f02\uff09\u3002<\/p>
\u539f\u56e0\uff1a\u83cc\u4f53\u5408\u6210\u5927\u91cf\u7ec6\u83cc\u53f6\u7eff\u7d20\uff08Bchl a\uff09\u548c\u7c7b\u80e1\u841d\u535c\u7d20\uff08\u5982\u87ba\u83cc\u9ec4\u7d20\uff09\uff0c\u7528\u4e8e\u5149\u5408\u4f5c\u7528\u3002<\/p>
\u900f\u660e\u5ea6\uff1a\u5747\u5300\u6d51\u6d4a\uff0c\u65e0\u660e\u663e\u6c89\u6dc0\u6216\u5206\u5c42\u3002<\/p>
\u5bf9\u6570\u751f\u957f\u671f\u540e\u671f\uff1a<\/p>
\u989c\u8272\u53ef\u80fd\u53d8\u6d45\uff1a\u56e0\u90e8\u5206\u83cc\u4f53\u8fdb\u5165\u7a33\u5b9a\u671f\uff0c\u4ee3\u8c22\u901f\u7387\u4e0b\u964d\uff0c\u8272\u7d20\u5408\u6210\u51cf\u5c11\u3002<\/p>
\u4e8c\u3001\u989c\u8272\u5f02\u5e38\u4e0e\u6f5c\u5728\u95ee\u9898\u5206\u6790<\/p>
1. \u989c\u8272\u53d8\u6d45\u6216\u53d1\u767d<\/p>
\u53ef\u80fd\u539f\u56e0\uff1a<\/p>
\u8425\u517b\u7f3a\u4e4f\uff1a\u78b3\u6e90\uff08\u5982\u4e59\u9178\u94a0\uff09\u6216\u6c2e\u6e90\uff08\u5982NH\u2084Cl\uff09\u8017\u5c3d\uff0c\u5bfc\u81f4\u83cc\u4f53\u505c\u6b62\u589e\u6b96\u3002<\/p>
\u5149\u7167\u4e0d\u8db3\uff1a\u5149\u5408\u8272\u7d20\u5408\u6210\u53d7\u963b\uff08\u9700\u5149\u7167\u5f3a\u5ea61000-5000 lux\uff0c\u6ce2\u957f800-900 nm\u8fd1\u7ea2\u5916\u5149\uff09\u3002<\/p>
\u6c27\u5316\u538b\u529b\uff1a\u57f9\u517b\u57fa\u6c27\u5316\u8fd8\u539f\u7535\u4f4d\u8fc7\u9ad8\uff08\u9700\u6dfb\u52a0\u534a\u80f1\u6c28\u9178\u3001Na\u2082S\u7b49\u8fd8\u539f\u5242\uff09\u3002<\/p>
\u6e29\u5ea6\u4e0d\u9002\uff1a\u8d85\u51fa\u6700\u9002\u751f\u957f\u6e29\u5ea6\uff0830-35\u2103\uff09\uff0c\u9ad8\u6e29\u5bfc\u81f4\u86cb\u767d\u8d28\u53d8\u6027\uff0c\u4f4e\u6e29\u6291\u5236\u4ee3\u8c22\u3002<\/p>
\u89e3\u51b3\u65b9\u6848\uff1a<\/p>
\u8865\u52a0\u65b0\u9c9c\u57f9\u517b\u57fa\u6216\u5173\u952e\u8425\u517b\uff08\u5982\u8865\u52a00.2%\u4e59\u9178\u94a0\uff09\u3002<\/p>
\u8c03\u6574\u5149\u6e90\u6ce2\u957f\u548c\u5f3a\u5ea6\uff0c\u4f7f\u7528\u7ea2\u5916LED\u706f\u6216\u767d\u70bd\u706f\u3002<\/p>
\u8865\u5145\u8fd8\u539f\u5242\uff08\u59820.05% Na\u2082S\u00b79H\u2082O\uff09\u5e76\u901a\u6c2e\u6c14\u9a71\u6c27\u3002<\/p>
\u4e25\u683c\u63a7\u6e29\u572830-35\u2103\u8303\u56f4\u5185\u3002<\/p>
2. \u989c\u8272\u53d8\u7eff\u6216\u84dd\u7eff\u8272<\/p>
\u53ef\u80fd\u539f\u56e0\uff1a<\/p>
\u6742\u83cc\u6c61\u67d3\uff1a\u7eff\u786b\u7ec6\u83cc\uff08\u5982Chlorobium\uff09\u6216\u84dd\u7ec6\u83cc\uff08\u5982Synechocystis\uff09\u7ade\u4e89\u6027\u751f\u957f\u3002<\/p>
\u5149\u8d28\u4e0d\u5339\u914d\uff1a\u4f7f\u7528\u77ed\u6ce2\u957f\u5149\u6e90\uff08\u5982\u84dd\u5149\uff09\u8bf1\u5bfc\u5f02\u5e38\u8272\u7d20\u8868\u8fbe\u3002<\/p>
\u89e3\u51b3\u65b9\u6848\uff1a<\/p>
\u955c\u68c0\u786e\u8ba4\u6c61\u67d3\uff08\u7eff\u786b\u7ec6\u83cc\u5448\u6746\u72b6\u3001\u84dd\u7ec6\u83cc\u6709\u5149\u5408\u7247\u5c42\uff09\uff0c\u66f4\u6362\u57f9\u517b\u57fa\u5e76\u4e25\u683c\u706d\u83cc\uff08121\u2103 20\u5206\u949f\uff09\u3002<\/p>
\u4f7f\u7528\u957f\u6ce2\u957f\u5149\u6e90\uff08\uff1e800 nm\uff09\u6216\u6dfb\u52a0\u6ee4\u5149\u7247\u3002<\/p>
3. \u989c\u8272\u53d8\u68d5\u9ec4\u6216\u7070\u8910\u8272<\/p>
\u53ef\u80fd\u539f\u56e0\uff1a<\/p>
\u4ee3\u8c22\u4ea7\u7269\u79ef\u7d2f\uff1a\u786b\u5316\u7269\uff08\u5982H\u2082S\uff09\u6216\u6709\u673a\u9178\u8fc7\u91cf\u5bfc\u81f4pH\u4e0b\u964d\uff08\u7406\u60f3pH 6.8-7.5\uff09\u3002<\/p>
\u83cc\u4f53\u81ea\u6eb6\uff1a\u957f\u671f\u57f9\u517b\u672a\u4f20\u4ee3\uff0c\u83cc\u4f53\u6b7b\u4ea1\u540e\u91ca\u653e\u80de\u5185\u7269\u8d28\u3002<\/p>
\u89e3\u51b3\u65b9\u6848\uff1a<\/p>
\u76d1\u6d4b\u5e76\u8c03\u8282pH\uff08\u6dfb\u52a01M NaOH\u6216\u7f13\u51b2\u6db2\u5982HEPES\uff09\u3002<\/p>
\u7f29\u77ed\u57f9\u517b\u5468\u671f\uff0c\u53ca\u65f6\u8f6c\u63a5\u65b0\u9c9c\u57f9\u517b\u57fa\uff08\u6bcf3-5\u5929\u4f20\u4ee3\u4e00\u6b21\uff09\u3002<\/p>
4. \u989c\u8272\u53d1\u9ed1\u6216\u6709\u9ed1\u8272\u6c89\u6dc0<\/p>
\u53ef\u80fd\u539f\u56e0\uff1a<\/p>
\u786b\u5316\u7269\u6c89\u6dc0\uff1a\u8fc7\u91cf\u786b\u6e90\uff08\u5982Na\u2082S\uff09\u5728\u9178\u6027\u6761\u4ef6\u4e0b\u751f\u6210FeS\u6216MnS\u9ed1\u8272\u6c89\u6dc0\u3002<\/p>
\u91cd\u91d1\u5c5e\u6bd2\u6027\uff1a\u57f9\u517b\u57fa\u4e2dFe\u00b2\u207a\u3001Cu\u00b2\u207a\u7b49\u79bb\u5b50\u6d53\u5ea6\u8fc7\u9ad8\u3002<\/p>
\u89e3\u51b3\u65b9\u6848\uff1a<\/p>
\u964d\u4f4e\u786b\u6e90\u6d53\u5ea6\uff0c\u63a7\u5236pH\u57287.0\u4ee5\u4e0a\u9632\u6b62\u786b\u5316\u7269\u6790\u51fa\u3002<\/p>
\u4f7f\u7528\u53bb\u79bb\u5b50\u6c34\u914d\u5236\u57f9\u517b\u57fa\uff0c\u6dfb\u52a0EDTA\uff080.01-0.1 mM\uff09\u87af\u5408\u91cd\u91d1\u5c5e\u3002<\/p>
\u4e09\u3001\u989c\u8272\u53d8\u5316\u7684\u5206\u5b50\u673a\u5236\u4e0e\u6df1\u5c42\u89e3\u6790<\/p>
1. \u5149\u5408\u8272\u7d20\u5408\u6210\u7684\u8c03\u63a7<\/p>
\u7ec6\u83cc\u53f6\u7eff\u7d20\uff08Bchl a\uff09\u4e0e\u7c7b\u80e1\u841d\u535c\u7d20\u7684\u5e73\u8861<\/p>
\u6b63\u5e38\u7ea2\u8272\u6e90\u4e8eBchl a\uff08\u5438\u6536\u8fd1\u7ea2\u5916\u5149\uff09\u4e0e\u7c7b\u80e1\u841d\u535c\u7d20\uff08\u5438\u6536\u84dd\u7eff\u5149\uff0c\u8d77\u5149\u4fdd\u62a4\u4f5c\u7528\uff09\u7684\u534f\u540c\u8868\u8fbe\u3002<\/p>
\u989c\u8272\u53d8\u6d45\uff1a\u53ef\u80fd\u56e0\u5149\u7167\u8fc7\u5f3a\u5bfc\u81f4\u7c7b\u80e1\u841d\u535c\u7d20\u5360\u6bd4\u5347\u9ad8\uff08\u83cc\u4f53\u542f\u52a8\u5149\u4fdd\u62a4\u673a\u5236\uff09\uff0c\u6216\u6c2e\u6e90\u4e0d\u8db3\u6291\u5236Bchl\u5408\u6210\u3002<\/p>
\u989c\u8272\u53d1\u7eff\uff1a\u82e5\u6c61\u67d3\u84dd\u7ec6\u83cc\uff08\u542b\u53f6\u7eff\u7d20a\uff09\u6216\u7eff\u786b\u7ec6\u83cc\uff08\u542bBchl c\/d\uff09\uff0c\u5149\u8c31\u5438\u6536\u5cf0\u504f\u79fb\u81f3\u53ef\u89c1\u5149\u533a\uff08\u5982680 nm\uff09\u3002<\/p>
2. \u6c27\u5316\u8fd8\u539f\u72b6\u6001\u5bf9\u8272\u7d20\u7684\u5f71\u54cd<\/p>
\u57f9\u517b\u57fa\u4e2d\u8fd8\u539f\u5242\uff08\u5982Na\u2082S\uff09\u4e0d\u8db3\u65f6\uff0cEh\uff08\u6c27\u5316\u8fd8\u539f\u7535\u4f4d\uff09\u5347\u9ad8\uff0c\u6291\u5236\u5149\u5408\u819c\u7cfb\u7edf\u53d1\u80b2\uff0c\u5bfc\u81f4Bchl\u5408\u6210\u53d7\u963b\u3002<\/p>
\u68c0\u6d4b\u5efa\u8bae\uff1a\u4f7f\u7528\u94c2\u7535\u6781\u5b9e\u65f6\u76d1\u6d4bEh\u503c\uff0c\u76ee\u6807\u8303\u56f4-150\uff5e-250 mV\uff08\u538c\u6c27\u5149\u5408\u4ee3\u8c22\u7684\u5178\u578b\u503c\uff09\u3002<\/p>
3. \u786b\u4ee3\u8c22\u4e0e\u989c\u8272\u5f02\u5e38<\/p>
\u6cbc\u6cfd\u7ea2\u5047\u5355\u80de\u83cc\u53ef\u5229\u7528\u786b\u5316\u7269\uff08S\u00b2\u207b\uff09\u4f5c\u4e3a\u7535\u5b50\u4f9b\u4f53\uff0c\u4f46\u8fc7\u91cf\u786b\u5316\u7269\u4f1a\u5bfc\u81f4\uff1a<\/p>
\u9ed1\u8272\u6c89\u6dc0\uff1aS\u00b2\u207b\u4e0eFe\u00b2\u207a\u751f\u6210FeS\uff08\u57f9\u517b\u57fa\u542b\u94c1\u65f6\uff09\u3002<\/p>
pH\u9aa4\u964d\uff1a\u786b\u6c27\u5316\u4ea7\u751fH\u2082SO\u2084\uff0c\u6291\u5236\u83cc\u4f53\u751f\u957f\uff08\u989c\u8272\u53d8\u9ec4\u8910\uff09\u3002<\/p>
\u5bf9\u7b56\uff1a\u63a7\u5236\u786b\u6e90\u6d53\u5ea6\uff08\u22641 mM\uff09\uff0c\u6dfb\u52a0pH\u7f13\u51b2\u5242\uff08\u598210 mM MOPS\uff09\u3002<\/p>
\u56db\u3001\u9ad8\u7ea7\u57f9\u517b\u4f18\u5316\u7b56\u7565<\/p>
1. \u52a8\u6001\u8865\u6599\u57f9\u517b<\/p>
\u95ee\u9898\uff1a\u5206\u6279\u57f9\u517b\u4e2d\u78b3\u6e90\uff08\u5982\u4e59\u9178\uff09\u5feb\u901f\u8017\u5c3d\uff0c\u5bfc\u81f4\u751f\u957f\u505c\u6ede\uff08\u989c\u8272\u53d8\u6d45\uff09\u3002<\/p>
\u89e3\u51b3\u65b9\u6848\uff1a<\/p>
\u91c7\u7528\u6052\u5316\u5668\uff08Chemostat\uff09\u8fde\u7eed\u57f9\u517b\uff0c\u7ef4\u6301\u78b3\u6c2e\u6bd4\u7a33\u5b9a\u3002<\/p>
\u8865\u6599\u7b56\u7565\uff1a\u5f53OD660\u8fbe\u52300.8\u65f6\uff0c\u6d41\u52a00.5%\u4e59\u9178\u94a0\uff08\u6bcf\u5c0f\u65f6\u8865\u52a0\u4f53\u79ef\u76841%\uff09\u3002<\/p>
2. \u5149\u751f\u7269\u53cd\u5e94\u5668\u8bbe\u8ba1<\/p>
\u5149\u8def\u4f18\u5316\uff1a<\/p>
\u4f7f\u7528\u5149\u7ea4\u5bfc\u5149\u7cfb\u7edf\uff0c\u786e\u4fdd\u57f9\u517b\u6db2\u5185\u90e8\u5149\u5747\u5300\u5206\u5e03\uff08\u907f\u514d\u8868\u9762\u5149\u8fc7\u5f3a\u800c\u5e95\u5c42\u5149\u4e0d\u8db3\uff09\u3002<\/p>
\u5149\u6ce2\u957f\u9009\u62e9\uff1a\u4f18\u5148\u4f7f\u7528850-900 nm LED\uff08\u5339\u914dBchl a\u5438\u6536\u5cf0\uff09\u3002<\/p>
\u63a7\u6c27\u6280\u672f\uff1a<\/p>
\u5fae\u597d\u6c27\u6761\u4ef6\u4e0b\uff0c\u901a\u5165N\u2082:CO\u2082=95:5\u6df7\u5408\u6c14\u4f53\uff0c\u7ef4\u6301\u6eb6\u89e3\u6c27\uff1c0.1 mg\/L\uff08\u907f\u514d\u5149\u5408\u7cfb\u7edf\u6c27\u5316\u635f\u4f24\uff09\u3002<\/p>
3. \u4ee3\u8c22\u7ec4\u5b66\u8f85\u52a9\u8bca\u65ad<\/p>
\u82e5\u989c\u8272\u5f02\u5e38\u4e14\u5e38\u89c4\u65b9\u6cd5\u65e0\u6548\uff0c\u53ef\u68c0\u6d4b\u57f9\u517b\u6db2\u4e2d\u4ee3\u8c22\u7269\uff1a<\/p>
\u6db2\u76f8\u8272\u8c31\uff08HPLC\uff09\uff1a\u5206\u6790\u4e59\u9178\u3001\u4e19\u9178\u7b49\u6709\u673a\u9178\u6b8b\u7559\u91cf\uff0c\u5224\u65ad\u78b3\u6e90\u5229\u7528\u7387\u3002<\/p>
\u6c14\u76f8\u8272\u8c31\uff08GC\uff09\uff1a\u68c0\u6d4bH\u2082S\u3001CO\u2082\u7b49\u6c14\u4f53\u4ee3\u8c22\u4ea7\u7269\uff0c\u8bc4\u4f30\u786b\u4ee3\u8c22\u662f\u5426\u5931\u8861\u3002<\/p>
\u4e94\u3001\u7591\u96be\u6848\u4f8b\u89e3\u6790<\/p>
\u6848\u4f8b1\uff1a\u83cc\u6db2\u989c\u8272\u53cd\u590d\u53d8\u767d<\/p>
\u73b0\u8c61\uff1a\u63a5\u79cd\u540e\u521d\u671f\u5448\u7ea2\u8272\uff0c3\u5929\u540e\u53d8\u767d\uff0c\u8865\u52a0\u8425\u517b\u540e\u6062\u590d\uff0c\u4f46\u5faa\u73af\u51fa\u73b0\u3002<\/p>
\u8bca\u65ad\uff1a<\/p>
\u955c\u68c0\u53d1\u73b0\u83cc\u4f53\u805a\u96c6\u6210\u56e2\uff08\u751f\u7269\u819c\uff09\uff0c\u5bfc\u81f4\u4f20\u4ee3\u4e0d\u5747\u3002<\/p>
\u68c0\u6d4b\u53d1\u73b0\u57f9\u517b\u57fa\u4e2dMg\u00b2\u207a\u4e0d\u8db3\uff08\uff1c0.1 mM\uff09\uff0c\u5f71\u54cd\u7ec6\u80de\u819c\u7a33\u5b9a\u6027\u3002<\/p>
\u89e3\u51b3\uff1a<\/p>
\u6dfb\u52a05 mM MgSO\u2084\uff0c\u5e76\u52a0\u51650.01% Tween 80\u5206\u6563\u83cc\u4f53\u56e2\u805a\u3002<\/p>
\u6539\u7528\u78c1\u529b\u6405\u62cc\u57f9\u517b\uff08\u8f6c\u901f50 rpm\uff09\u589e\u5f3a\u4f20\u8d28\u3002<\/p>
\u6848\u4f8b2\uff1a\u989c\u8272\u5448\u5f02\u5e38\u7d2b\u9ed1\u8272<\/p>
\u73b0\u8c61\uff1a\u83cc\u6db2\u5448\u7d2b\u9ed1\u8272\uff0c\u79bb\u5fc3\u540e\u4e0a\u6e05\u4e3a\u9ec4\u8272\uff0c\u6c89\u6dc0\u4e3a\u9ed1\u8272\u9897\u7c92\u3002<\/p>
\u8bca\u65ad\uff1a<\/p>
X\u5c04\u7ebf\u884d\u5c04\uff08XRD\uff09\u663e\u793a\u6c89\u6dc0\u542bFe\u2083O\u2084\uff08\u78c1\u94c1\u77ff\uff09\uff0c\u56e0\u57f9\u517b\u57fa\u4e2dFe\u00b2\u207a\u8fc7\u91cf\uff08\uff1e5 mM\uff09\u4e14pH\uff1e7.5\u65f6\u81ea\u53d1\u6c27\u5316\u3002<\/p>
\u89e3\u51b3\uff1a<\/p>
\u964d\u4f4eFeSO\u2084\u6d53\u5ea6\u81f30.1 mM\uff0c\u6dfb\u52a010 mM EDTA\u87af\u5408\u6e38\u79bbFe\u00b2\u207a\u3002<\/p>
\u63a7\u5236pH\u57287.0\u4ee5\u4e0b\uff08\u901a\u8fc7\u8c03\u8282CO\u2082\u901a\u6c14\u6bd4\u4f8b\uff09\u3002<\/p>
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1. \u9632\u6b62\u83cc\u79cd\u9000\u5316\u7684\u63aa\u65bd<\/p>
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