BBM培養(yǎng)基——Bold’s?Basal?Medium(Deason?&?bold?1960;Bischoff?&?Bold?1963)
NaNO3??????????250mg????????KH2PO4????????175mg
K2HPO4?????????75mg?????????MgSO4·7H2O????75mg
CaCl2·2H2O??????25mg?????????NaCl???????????25?m
EDTA???????????50mg?????????KOH???????????31mg
FeSO4·7H2O??????4.98mg???????H3BO3??????????11.42mg
ZnSO4·7H2O??????8.82mg???????MnCl2??????????1.44mg
MoO3????????????0.71mg???????CuSO4·5H2O????1.57?mg
Co(NO3)2?·6H2O??0.49mg???????加蒸餾水補(bǔ)足1000ml?
可以在以上組份中加入15~20g瓊脂,并蒸餾水補(bǔ)足1L制成固體培養(yǎng)基。
3×N?BBM培養(yǎng)基(Brown?&?Bold?1964)?
是由BBM改進(jìn)的培養(yǎng)基,只是比BBM多了3倍的氮素。?
同上一樣,只是NaNO3為750mg。如制成固體培養(yǎng)基,需在以上配方中加入15~20g瓊脂,并蒸餾水補(bǔ)充1L。