Media for freshwater, terrestrial, hot spring and salt water algae

C

Ca(NO3)2 · 4H2O 15 mg

KNO3 10 mg

β-Na2glycerophosphate · 5H2O 5 mg

MgSO4 · 7H2O 4 mg

Vitamin B12 0.01 μg

Biotin 0.01 μg

Thiamine HCl 1 μg

PIV metals 0.3 mL

Tris (hydroxymethyl) aminomethane 50 mg

Distilled water 99.7 mL

pH 7.5

Add 1.5 g agar to 100 mL of medium to give a solid medium.

Reference

Ichimura, T. 1971 Sexual cell division and conjugation-papilla formation in sexual reproduction of Closterium strigosum. In Proceedings of the Seventh International Seaweed Symposium, University of Tokyo Press, Tokyo, p. 208-214.

PIV metals

Na2EDTA · 2H2O 100 mg

FeCl3 · 6H2O 19.6 mg

MnCl2 · 4H2O 3.6 mg

ZnCl2 1) 1.04 mg

CoCl2 · 6H2O 0.4 mg

Na2MoO4 · 2H2O 0.25 mg

Distilled water 100 mL

1) In the NIES-Collection, 1.04 mg ZnCl2 is replaced by 2.2mg ZnSO4 · 7H2O.

Reference

Provasoli, L., Pintner, I. J. 1959 Artificial media for fresh-water algae: problems and suggestions. In The Ecology of Algae. Spec. Pub. No. 2,, Eds. by Tryon, C. A., Jr. & Hartmann, R. T., Pymatuning Laboratory of Field Biology, University of Pittsburgh, Pittsburgh, p. 84-96.